Molecular diversity analysis in Wheat genotypes using SSR markers

  • Pawan Kumar Yadav, CCS Haryana Agricultural University, Hisar
  • Ramesh Kumar Yadava, CCS Haryana Agricultural University, Hisar
  • Sandeep Kumar, CCS Haryana Agricultural University, Hisar
  • Pritam Kumar CCS Haryana Agricultural University, Hisar
Keywords: Wheat (Triticum aestivum L.), Molecular markers, Simple Sequence Repeats, genetic diversity

Abstract

Molecular diversity of the seven wheat (Triticum aestivum L.) genotypes was evaluated using 50 SSR primers.  Genetic  distances  were  calculated  using  unweighted  pair  group  of  arithmetic  mean (UPGMA) procedure. A high degree of genetic polymorphism was observed among the wheat varieties with average genetic polymorphism 70%.  SSR diversity data was generated using above primers and a total of 114 alleles were detected with an average of 2.71 alleles per locus. The number of alleles per locus ranged from 2 to 6 and the percent of polymorphism ranged from 20% for the Xwmc254 to 100% for the Xbarc26. The average genetic diversity based on SSR markers was 0.465 with a range of 0.037- 0.892. Our results suggested that the classification based on genotypic markers of these wheat genotypes would be useful for wheat breeders to plan crosses for positive traits. The results obtained suggested that the wheat microsatellite markers could be used to distinguish wheat genotypes and to estimate genetic diversity. The present study also indicates that microsatellite markers permit the fast and high throughout fingerprinting of accessions from a varieties collection in order to assess genetic diversity. 

Author Biographies

Pawan Kumar Yadav, CCS Haryana Agricultural University, Hisar
Genetics And Plant Breeding
Ramesh Kumar Yadava, CCS Haryana Agricultural University, Hisar
Ex- Head of Section, Department of Genetics and Plant Breeding
Sandeep Kumar, CCS Haryana Agricultural University, Hisar
Department of Genetics and Plant Breeding
Pritam Kumar, CCS Haryana Agricultural University, Hisar
Department of Molecular Biology and Biotechnology
Published
24-09-2016
How to Cite
Pawan Kumar Yadav, Ramesh Kumar Yadava, Sandeep Kumar, Pritam Kumar
Molecular diversity analysis in Wheat genotypes using SSR markers. 2016. Electronic Journal of Plant Breeding, 7 2, 464-468. Retrieved from https://www.ejplantbreeding.org/index.php/EJPB/article/view/1041
Issue
Vol 7 No 2 (2016)
Section
Research Note

It is certified that:

  • The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
  • The article has been seen by all the authors who are satisfied with its form and content.
  • The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
  • All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
  • The corresponding author takes full responsibility for this article.
  • The address of the organization where the research was conducted is given.
  • The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
  • Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
  • If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.