Molecular markers linked to fertility restorer gene in A2 cytoplasm male sterility of pigeon pea [Cajanus cajan]

  • Waseem Sheikh, Anavarali Dedhrotiya Nargish Khan, Avinash Rathod, Sampat Kalaskar and Suresh Acharya SARDARKRUSHINAGAR DANTIWADA AGRICULTURE UNIVERSITY
Keywords: C. cajan, C. scarabaeoides, CGMS system, RAPD, SSR

Abstract

In pigeonpea the cytoplasmic-genetic male sterility (CMS) system is considered to be  a feasible approach for the  development of  hybrids. Identification of CMS lines and their putative restorers using molecular markers in the early stage of growth is an important and economical in long duration pigeonpea. Based on phenotyping, the 120 plants of F2 designated as fertile and sterile. Both visual and pollen staining phenotyping unequivocally exhibited 85 fertile: 35 sterile plants in F2 population. The segregation of F2 for fertility restoration of A2 cytoplasm indicated 3F:1S segregation indicating dominant monogenic control of fertility restoration. Bulk segregant analysis revealed that 10 of the 139 polymorphic decamer RAPD markers produced precisely distinct bands subjective to fertility restorer DNA bulk while, none of the SSR was found polymorphic between bulks. Two of them viz; OPC 7617 (GTC CCG ACG A) and OPK 3860 (CCA GCT TAG G) evinced clear and unambiguous bands for fertility restorations in F2 population at 0.5 and 1.0 cM, respectively. They could be exploited as a precise selection tool for seminal but stubborn character like restoration of fertility in male sterile lines that has germane significance in otherwise immensely important heterosis breeding in pigeonpea.

 

Key Words

C. cajan, C. scarabaeoides, CGMS system, RAPD, SSR

Published
24-01-2017
Section
Research Article