Assessment of molecular diversity in an elite set of finger millet (Eleusine coracana (L.) Gaertn.) genotypes using SSR markers

  • Manjappa, Santosh Deshpande, S. Rangaiah and M.V.C. Gowda Central Tasar Research and Training Institute, Ranchi
Keywords: Finger millet, molecular diversity, SSR marker, AMOVA, cluster

Abstract

Genetic diversity was assessed among 47 elite finger millet genotypes (29 varieties and 18 African and Asian germplasm) using 16 SSR markers. Owing to polyploidy nature of crop seven SSR markers have amplified more than one dose of alleles per genotype.  Thirteen markers were polymorphic with polymorphic information content from 0.02 (UGEP56) to 0.61 (UGEP64) with an average of 0.22. A total of 72 alleles were detected across genotypes and alleles per SSR marker loci ranged from 1 to 14 (UGEP107) with an average of 4.69. UPGMA based dendrogram generated by Jaccard’s similarity coefficient had grouped 47 genotypes in to three clusters and they were widely distributed on two dimension principle component analysis graph. Analysis of molecular variance revealed higher polymorphism in varieties (53.39 %) than in germplasm (39.71 %), implying higher genetic variability in cultivated varieties. The information gathered in the study will be useful in planning hybridization for further finger millet improvement.

 

Author Biography

Manjappa, Santosh Deshpande, S. Rangaiah and M.V.C. Gowda, Central Tasar Research and Training Institute, Ranchi
Silkworm Breeding and Genetics
Published
09-07-2018
Section
Research Article