Simple and quick method of DNA extraction from different parts of plant for PCR amplification

  • S. Kiruthika and B. V. Padmanabha OrrisLife Sciences Pvt. Ltd.
Keywords: DNA extraction, SDS (Sodium dodecyl sulphate), NaCl (Sodium chloride), PVP(Polyvinylpyrrolidone), PCR (Polymerase chain Reaction)

Abstract

Various protocols are usually used for extracting genomic DNA from plants for genetic analysis. Majority of currently used DNA extraction methods and commercially available kits are multi-stepped, time consuming, expensive especially when dealing with large number of samples. In the present study, we have developed a universal method for extracting DNA from various parts of different plants which is inexpensive, quick and easy. In this method, to extract the DNA, 0.5% SDS, 0.5 M NaCl, 0.5% PVP and 100mM Tris HCl (pH 8.0) were used in the buffer. Leaf samples were ground with 50µL of buffer in 1.5 ml tube and was stabilised with 1000µL of 100mM Tris-HCl buffer. 2µL of lysate from this extract was used for setting PCR. The method doesn’t require hazardous chemicals or purification procedures. The DNA extracted was found to be sufficient and suitable for PCR amplification with high reproducibility.

Author Biography

S. Kiruthika and B. V. Padmanabha, OrrisLife Sciences Pvt. Ltd.

Department of Research and Development

Research Associate

Published
03-04-2018
Section
Research Note