Development of a simple and genotype independent in vitro regeneration system in Sugarcane [Saccharum spp] using shoot apex explants
Sugarcane (Saccharum spp) is commercially cultivated in Tamil Nadu for industrial purposes. Germplasm of this crop is mainly conserved in the form of clonal gene bank. For long term conservation of vegetatively propagated crops, cryo-preservation offers a promising approach which could be realized through a reliable and genotype independent tissue culture system. In the present study, two sugarcane genotypes namely Co 86032 and TN SC Si 7 were evaluated for their in vitro regeneration response through optimization of various factors influencing organogenesis. The results indicated that shoot apices explants inoculated on medium supplemented with 0.25 mg/l BAP + 5.0 mg/l kinetin + 0.05 mg/l NAA recorded highest shoot induction frequencies of 92.2% and 84.4% for Co 86032 and TN SC Si 7 respectively. On an average, 6 to 8 shoots were formed per explant. After 60 days of culture, single shoots were separated from the multiple shoots and transferred to the rooting medium. Among the different media tested, highest regeneration frequencies of 63.3% (Co 86032) and 56.6% (TN Sc Si 7) were found on medium supplemented with 5 mg/l NAA. Thus, a simple and genotype independent in vitro regeneration system was developed which could be well adopted for conservation of sugarcane germplasm in the cryo gene bank.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.