Induced genetic variability with EMS and studies on frequency and spectrum of chlorophyll mutations in Pigeonpea

  • Sandeep Kumar Saroj Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi- 221005
  • P. P. Poudel Department of Genetic and Plant Breeding, Institute of Agricultural Sciences, Varanasi-221
  • M. N. Singh Department of Genetic and Plant Breeding
Keywords: Pigeonpea, EMS, Chlorophyll mutation.

Abstract

The 400 pure seeds per treatments of each of two genotypes namely, MA 156 and MAL 13 of long duration pigeonpea (Cajanus cajan (L.) Millsp.) were treated with three different concentrations (0.01M, 0.015M and 0.02M) of EMS prepared in phosphate buffer to observed mutation. At lab condition germination varied from 71% in MAL 13 (at 0.02M) to 88% in MA 156 (at 0.01M). Average root and shoot length was highest at lower dose (0.01M) and lowest at higher doses (0.02M) in both the genotypes. Based on high fertility more than 85% showing 50 plants in each treatment were harvested separately and used to raising M2 generation. Among 6705 seedlings were observed in M2 population the highest in frequency of chlorophyll mutants (3.31%) at 0.015 M in MAL 13, followed by 2.73% at 0.015M in MA 156 and lowest (0.72%) was found at 0.01M in MA 156. Mutagenic efficiency and Mutagenic effectiveness were higher at 0.015M in MAL 13. Chlorophyll mutations include Albino, Xantha, Chlorina and Viridis.

Author Biographies

Sandeep Kumar Saroj, Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi- 221005
Institute of Agricultural Sciences
P. P. Poudel, Department of Genetic and Plant Breeding, Institute of Agricultural Sciences, Varanasi-221
Institute of Agricultural Sciences
M. N. Singh, Department of Genetic and Plant Breeding
Institute of Agricultural Sciences
Published
12-09-2016
Section
Research Article