SSR marker based DNA fingerprinting and diversity studies in mustard (Brassica juncea)

  • Puranjaya Panigrahi, Kaushik Kumar Pani and Somnath Bhattacharya Orissa University of Agriculture and Technology

Abstract

Seventeen mustard cultivars were subjected to SSR assay to identify the efficient primers for developing DNA fingerprinting. Two SSR primers of mustard Ra1H11 and Ra1F03 revealed a considerable DNA polymorphism of 41.17% and 35.29% respectively. The SSR primer Ra1H11 (0.772) of mustard had showed highest discriminating power followed by Ra1F09 (0.617) then Ra1F03 (0.558), Ra2B02 (0.382) and Ra1G07 (0.228) respectively. The highest PIC value had showed by Ra1F03 (0.525), then followed by), Ra1H11 (0.363), Ra2B02 (0.359), Ra1F09 (0.290) respectively. The primer Ra1H11 had showed highest MI value (0.423) then followed by Ra1F03 (0.262), Ra1F09 (0.193), Ra2B02 (0.119) and Ra1G07 (0.071) respectively. Among all the mustard SSR primers Ra1H11, Ra1F09, Ra1F03 followed a descending order of their efficiency to discriminate the mustard cultivars so that they can be used for developing DNA finger printing patterns for these cultivars. A combination of SSR primer Ra1F03 with Ra1F09 had distinguished the cultivar Bhagirathi from Panchali and Sanjukta, Ra1F09 with Ra1H11 had distinguished the cultivar kranti from Agrani and PT-303, Ra1F03 with Ra1F09 had distinguished the cultivar JD-6 from Pusa Bold, and Ra1F03 with Ra1H11 had distinguished the cultivar YST-151 from Jhumuka by producing a unique banding pattern

Author Biography

Puranjaya Panigrahi, Kaushik Kumar Pani and Somnath Bhattacharya, Orissa University of Agriculture and Technology
Assistant Professor
Department of Plant Breeding and Genetics
Orissa University of Agriculture and Technology
Bhubaneswar
Orissa.
Published
04-04-2018
Section
Research Article