Marker-assisted selection for biotic stress (Bacterial leaf blight and gall midge) tolerance in Bc4F4 generation of rice (Oryza sativa L.)
Bacterial blight (BB) of rice caused by the pathogen Xanthomonas oryzae pv. oryzae and the insect gall midge (GM) (Orseolia oryzae) are two major limitations of rice production. The present study was carried out to improve CO 43 a popular variety of Tamil Nadu for BB and GM resistance through marker assisted backcross breeding. Two sets of genes Xa21 and xa13 for bacterial blight resistance and Gm1 and Gm4 for gall midge resistance were used for this study. A total of 95 plants in BC4F4 generation were genotyped with SSR markers RM1328, RM22550, xa13 prom and pTA248 for the genes Gm1, Gm4, xa13 and Xa21 respectively. Artificial screening for bacterial blight screening was conducted in glass house condition and gall midge screening was conducted in the field condition. The lines selected for bacterial blight and gall midge resistance genes recorded a high level of resistance than the recurrent parent CO43.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.