DNA fingerprinting of groundnut (Arachis hypogaea L.) varieties of Tirupati using SSR markers
Keywords: Groundnut, SSR markers, DNA fingerprinting, Polymorphism information content, Cluster analysis.
AbstractUnambiguous identification of varieties is important for registration and certification of newly released varieties. Molecular markers are powerful tools, which help in differentiating plant varieties at the DNA level and have been widely used for fingerprinting in a number of crop varieties. In the present study, a set of 12 groundnut varieties released from Regional Agricultural Research Station, Tirupati were fingerprinted employing SSR markers. A total of 300 SSR were screened and fifteen potential markers were employed for fingerprinting of groundnut varieties. The SSR markers generated alleles ranging from 2 to 7 with an average of four per locus. The polymorphism information content (PIC) values ranged from 0 to 0.85. The genotypic data from all the loci provided unique SSR allelic fingerprints which helped in varietal identification of groundnut. Core set of highly informative primers viz., PM 377, TC1A02, TC5A06 and GM1489 identified in this study has the potential to identify most of the groundnut varieties. Cluster analysis using SSR marker grouped 12 groundnut varieties into two major clusters. Finger printing of the groundnut genotypes provide information about phylogenetic relationships and assists groundnut breeders in varietal registration and protection of intellectual property rights.
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