Assessing the viability and storage life in pollen grains of rice (Oryza sativa L.)
In rice (Oryza sativa L.), the major cereal and staple food of millions in the world, hybridization programmes are very crucial for crop improvement. When the parents involved in crossing programme are varying in their flowering duration, storage of pollen grains for long time is highly advantageous for producing desirable hybrid combinations at any time. A study was undertaken to assess the viability and storage life of rice pollen grains through different storage methods. The anthers with pollen grains of the rice variety Uma, were collected from the field lab and transferred to various storage media. Aluminium foil and polythene bag were used as storage materials along with/without desiccators. Silica gel, Rice flour and raw rice were used as the desiccators. Samples packed in various storage media were kept under different conditions like Ambient, Refrigerator and Freezer. Afterwards, viability (%) of each sample was observed on 5th, 10th, 15th, 20th, 25th and 30th day of the storage using Acetocarmine dye method. The results after one month of storage indicated that pollen kept in Aluminium foil with silica in all the conditions showed better viability percentage, and the highest (87.24%) was in Freezer. Pollen grains had low viability in normal storage (without any media/dessicator) in all cases except ambient condition wherein, the lowest percentage of viability was noticed in Polythene bag without silica. This result indicates that the pollen storage in the Aluminium foil with silica gel in freezer condition is a highly effective and promising strategy for plant breeders and farmers, involved in plant breeding, hybridisation and genetic conservation programmes.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.