Agrobacterium-mediated transformation of brinjal (Solanum melongena L.) using fungal resistant gene

  • Shreedhar Ganapati Bhat, G. Arulananthu, G. Rajesh and N. Ramesh Government Arts College for Men, Krishnagiri

Abstract

The investigation standardized the protocol for Agrobacterium-mediated transformation system in Brinjal (Solanum melongena L), cultivar ‘Arka Neelkanth’ . Initially the regeneration system was standardized with regeneration medium (MS basal medium supplemented with 8.88 µM BAP + 1.14 µM IAA) by using 10-12 days old cotyledon explants. Transformation of the hevein, a gene responsible for fungal resistance. into cotyledon explants was carried out with Agrobacterium tumefaciens strain LBA4404 harbouring binary plasmid pGPTV/hevein (Plasmid Gus Plant Transformation Vector) with marker gene neomycin phosphotransferase (npt-II). Transformation efficiency showed a higher percentage in one day pre-cultured explants, infected with bacterial suspension of optical density 0.4 at OD600 nm with 1:1 dilution for 10min and 48hrs co-cultivation on regeneration medium in the dark. Transformed explants were cultured on selection medium. The PCR molecular confirmation of amplified npt-II gene from the genomic DNA of the transformed plant indicates the integration of the hevein gene in the brinjal plant.

Published
06-04-2020
How to Cite
and N. Ramesh, S. G. B. (2020). Agrobacterium-mediated transformation of brinjal (Solanum melongena L.) using fungal resistant gene. Electronic Journal of Plant Breeding, 11(01), 160-168. Retrieved from http://www.ejplantbreeding.org/index.php/EJPB/article/view/3448
Section
Research Article