Assessment of Genetic Diversity in Mulberry using Morphological and Molecular markers
The horizontal expansion of sericulture in traditional and non-traditional states has made it necessary to develop mulberry varieties specific to various agro-climatic zones. At present various improved varieties in use are mostly developed, involving exotic accessions as parents. An understanding of genetic diversity is must for selection of parental lines to gain vigor in hybrid. In the present study, an initiative was taken to assess the genetic diversity among mulberry (Morus alba) genotypes using morphological and PCR based molecular markers. The morphological aspects considered in the present study revealed high variability. The number of leaves per meter twig was highest in Tr-10 and Chakmajra (22). Maximum inter-nodal distance was recorded in variety MS-9 and Chinese White (5.7cm). Actual leaf area was highest in LUN-10 (225.38cm2). The fresh weight of 100 leaves was highest in Kokuso-27 (339.0g) and dry-weight as (101.5g). Maximum leaf length observed was 23.0 cm in V-1 and maximum width of 17.3 cm was recorded in Kokuso-27. For molecular assessment of genetic diversity, nine reproducible RAPD primers were used that revealed more than 97.22 % polymorphism percentage with 0.32 average PIC value. Finally, the cluster analysis based on UPGMA divided the varieties in two major groups. Based on morphological and molecular analysis, diverse varieties having better agronomic traits can be selected as the parental source in developing new hybrids. Moreover, the RAPD profiles of each variety obtained from molecular analysis can be used to depict the authenticity of a cultivar and confirmation of hybrid variety.
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.