Characterization of soybean genotypes based on morphological and molecular markers
The present investigation was carried out with 38 soybean genotypes during Kharif, 2020. The variability analysis was done using 10 quantitative and 11 qualitative characters. Twenty four SSR markers were used to study genetic relationships among the genotypes based on Jaccard’s coefficient of similarity. The analysis of variance revealed that there were significant variations for yield and yield attributing characters. Plant height, the branches per plant, the pods per plant and seed yield showed the highest coefficients of variability along with high heritability with a genetic advance as percentage of mean. Correlation and path coefficient analyses identified plant height, the branches per plant and the pods per plant traits identified as important traits for yield improvement in soybean. Euclidean distance based on qualitative characters indicated the genotypes DLSb-2 and JS 22-14 are most divergent, while quantitative characters based analysis revealed that the genotypes NRC 128 and TS20-5 are potential for use in breeding. Out of 24 soybean specific SSR primers, 19 were found to be polymorphic. The number of SSR allele per locus ranged from one to three with an average of 1.4 alleles per locus. DNA marker analysis revealed a range of diversity in the experimental materials with few potential markers for diversity analysis due to their high PIC values.
Keywords: Euclidean distance, Genetic variability, Path coefficient analysis, Soybean, SSR marker
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.