Targeted editing of OsSWEET13, a bacterial leaf blight susceptible gene in rice using CRISPR tool
Bacterial leaf blight (BLB) of rice is considered to be a disease of economic importance as the disease causes severe yield losses in all rice growing regions. Transcription activator like effector (TALE) molecules are produced by the pathogen, Xanthomonas oryzae pv. oryzae (Xoo) bind to the effector binding element (EBE) of the promoter of SWEET gene and activates transcription of SWEET genes, making the plant susceptible to the disease. Innate resistance to Xoo in certain rice genotypes is due to mutations in EBE present in the upstream regulatory region of SWEET genes. CRISPR–mediated targeted modification of susceptibility gene/promoter is an effective approach to develop BLB resistance in rice. The present study was an attempt to repress TALE triggered signalling via introducing indels in the EBE of OsSWEET13 gene in a local popular rice genotype, CO51 employing CRISPR/Cas9 mediated genome editing tool with a view to imparting BLB resistance. Agrobacterium-mediated transformation using immature embryos followed by regeneration resulted in four independent transformation events. Five plants representing three events were found to have one nucleotide deletion in the target sequence. These deletion mutations in EBE could potentially interfere with the binding of the corresponding TALE to confer resistance against certain strains of BLB.
Keywords: Rice, Bacterial Leaf Blight, SWEET13 gene, Effector Binding Element, CRISPR/Cas9.
Targeted editing of OsSWEET13, a bacterial leaf blight susceptible gene in rice using CRISPR tool. Electronic Journal of Plant Breeding, 13 3, 772-779. Retrieved from https://www.ejplantbreeding.org/index.php/EJPB/article/view/4431
It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.