An insight on molecular diversity in ricebean (Vigna umbellata) genotypes using microsatellite markers
Abstract
A total of 25 adzukibean derived SSR markers were used for molecular diversity analysis among 45 ricebean genotypes and a greengram cultivar, CO 7. Cross species amplification was observed for all the markers in ricebean. Number of alleles, in the present study, varied from one to three. In ricebean, the allele size ranged from 110 to 250 bp, whereas in greengram cultivar, it ranged from 110 to 250 bp. PIC values ranged from 0.198 to 0.689. Out of 25 markers, 13 markers were found to be polymorphic among ricebean genotypes. Apart from this, 13 markers differentiated the ricebean genotypes from the greengram cultivar, CO 7. Dissimilarity values between the ricebean genotypes and the greengram cultivar, CO 7 were greater when compared to the dissimilarity values within the ricebean genotypes. Dendrogram and the tree generated based on Ward’s and neighbor joining methods classified the genotypes into five clusters and three groups, respectively.
Keywords: SSR, rice bean, diversity, cross species amplification
An insight on molecular diversity in ricebean Vigna umbellata genotypes using microsatellite markers
. 2023. Electronic Journal of Plant Breeding, 14 2, 521-529. Retrieved from https://www.ejplantbreeding.org/index.php/EJPB/article/view/4692It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.
