Biochemical analysis of metabolites in cotton (Gossypium hirsutum L.) conferring resistance to Leaf hopper Amrasca biguttula biguttula (Ishida)
DOI: 10.37992/2023.1403.108
Abstract
The cotton leafhopper Amrasca biguttula biguttula (Ishida) has emerged as a major threat, causing significant yield losses (40-60%). To address this, we aimed to identify cotton genotypes with superior biochemical traits for leafhopper resistance. Various biochemical parameters and elemental composition by EDAX-spectroscopy were analyzed. Field and polyhouse screenings were conducted for host plant resistance (HPR) study. The ANOVA, correlation, and stepwise regression analyses were conducted to assess the traits relationship and their impact on leafhopper resistance. Results showed significant genotype variations (p < 0.05) were observed in biochemical traits and leafhopper injury index. Two verities NDLH1938 and Suraksha and three F1 hybrids derived from crosses viz., TVH002 × Suraksha, NDLH1938 × Suraksha, and TVH002 × NDLH1938 were showing significant resistance by recording lower leafhopper population (2.28-5.17), injury index (0.69-0.97), susceptibility index (1.58-5.00) and host preference survival rate (2.21-2.46). The HPR study suggests that lines are less preferred by the leafhoppers to survive which indicates factors influencing antibiosis. Biochemical profiling revealed that gossypol (r = -0.75), tannins (r = -0.69), and calcium (r = -0.78) were negatively correlated with pest density. It indicates the genotypes with higher levels of gossypol, tannins, and calcium, and lower levels of reducing sugars, total proteins, free amino acids, and chlorophyll exhibited resistance to the leafhopper. Stepwise regression analysis indicated the combined effect of these biochemical traits (R2 = 0.93) in contributing to a high-level of resistance rather than individual traits. Our findings highlight the importance of biochemical traits in influencing leafhopper resistance and the need for comprehensive trait combinations in breeding resistant cotton varieties.
Keywords: Breeding, cotton, correlation, gossypol, injury index, leafhopper resistance, tannins, regression.
Biochemical analysis of metabolites in cotton Gossypium hirsutum L. conferring resistance to Leaf hopper Amrasca biguttula biguttula Ishida
. 2023. Electronic Journal of Plant Breeding, 14 3, 965-975. Retrieved from https://www.ejplantbreeding.org/index.php/EJPB/article/view/4824It is certified that:
- The corresponding author is fully responsible for any disputes arising due to the publication of his/her manuscript.
- The article has been seen by all the authors who are satisfied with its form and content.
- The sequence of names of authors in the by-line is as per their relative contribution to this experiment, giving due credit to all scientists who made notable contribution to it.
- All the authors fully understand that inclusion of any other co-authors or exclusion of any co-authors is not possible once the article has been submitted to the journal.
- The corresponding author takes full responsibility for this article.
- The address of the organization where the research was conducted is given.
- The article is exclusive for this journal, and the results reported here have not been sent (and will not be sent during its consideration by this journal) for publication in any other journal.
- Authors agree to abide by the objective comments of referees and do agree to modify the article into a short note as per the recommendation, for publication in the Electronic Journal of Plant Breeding.
- If published in Electronic Journal of Plant Breeding, the copyright of this article would vest with the Indian Society of Plant Breeders, who will have the right to enter into any agreement with any organization in India or abroad engaged in reprography, photocopying, storage and dissemination of information contained in it, and neither we nor our legal heirs will have any claims on royalty.
