Assessment of molecular diversity in elite Sweet Orange (Citrus Sinensis L.Osback) accessions using RAPD Markers

  • Madhumathi Chinthagunta Horticultural Research Station, Anantharajupet, Kodur - 516 105 Dr YSR Horticultural University Andhra Pradesh
  • K . Purushotham Dr. YSR Horticultural University, Venkatrammanagundem
  • R . Chandrasekhar College of Horticulture, Mojerrlla.
  • K . Gopal Horticulture and Research Institute, Anantharajupet, Kodur.
  • M. Reddi Sekhar S.V. Agricultural College, Tirupathi.
  • T. Gowri Shankar Citrus Research Station, Tirupathi.
Keywords: Sweet orange, genetic diversity, germplasm analysis, RAPDs

Abstract

Random amplified polymorphic DNA (RAPD) markers were used to estimate the  genetic diversity among 27  elite sweet orange accessions of Citrus Research Station (AICRP on citrus), Tirupati. The 52 decamer primers generated 469 randomly amplified DNA fragments, of which 292 were polymorphic (62.26%). The similarity indices measured based on Jaccard similarity coefficient ranged from 0.52 to 0.80, which showed the presence of low to moderate diversity among twenty seven sweet orange accessions.  Based on UPGMA cluster analysis, 27accessions were classified into two main clusters.  All 26 sweet orange accessions formed a major cluster.  HS-3 accession having its clear genetic identity formed another cluster with the rest of the sweet orange accessions.  The sweet orange accessions with great economic importance viz.,Jaffa, Blood RedMalta, Mediterranean Sweet, Hamlin Sweet, Valencia Late, Pineapple and Kodur Sathgudi showed complete genetic similarity.  RAPD analysis showed that most of the accessions of sweet orange were closely clustered, with relatively high genetic similarity (0.75), suggesting that the genetic base of domesticated sweet orange is quite narrow. 

Author Biography

Madhumathi Chinthagunta, Horticultural Research Station, Anantharajupet, Kodur - 516 105 Dr YSR Horticultural University Andhra Pradesh

Department of fruit Science

 

 

 

 

Published
24-09-2016
Section
Research Article