Callus induction in Hydnocarpus pentandra (Buch. Ham.) Oken using shoot tips

Vighnesha Bhat
P. E. Rajasekharan
G. Raviraja Shetty
Sadashiv Nadukeri
M. Ganapati
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Abstract

Hydnocarpus pentandra (Buch. Ham.) Oken is a Vulnerable medicinal tree found in Western Ghats ofIndia. It is highly exploited due to its medicinal properties and it is mainly used in leprosy treatment. Anexperiment was undertaken at Division of Plant Genetic Resources, ICAR-Indian Institute of HorticulturalSciences, Bengaluru during 2018-19. The experiment was laid down in Completely Randomized Design(CRD) with 16 treatments and three replications. Shoot tips from young seedlings were used as explantand treated in MS media with a different hormonal combination like Benzyl Amino Purine (BAP), IndoleAcetic Acid (IAA), Kinetin and Thiaduzerol (TDZ). Among the treatments, highest shoot length (2.06 cm)and maximum number of shoots produced per explant (3.86) were found in the treatment combination ofBAP (1.0 mg/l) and TDZ (0.5 mg/l) followed by the treatment combination of BAP (2.4 mg/l) and IAA (0.05mg/l). Few treatments showed callus formation but no shoots were developed in these treatments.

Keywords Vulnerable medicinal tree, Growth hormones, Explant, Callus induction
Published 09/02/26