Screening of sodicity tolerant and to survey parental polymorphism in CSR 11 X MI 48 cross using SSR markers

Abstract

Rice (Oryza.sativa.L) is adapted to all types of soils. Soils in semi-arid or arid regions, especially those with ineffective drainage, accumulate salt as irrigation water evaporates leaving behind deposits of soluble salts. Sodic soil is the one that has been adversely affected by sodium. Sodium toxicity represents the major ionic stress associated with high salinity. Rice can be grown in alkali soils having pH upto 9.6, but in soils having higher pH, alkali tolerant varieties should be grown or some amendment should be done to reduce the pH upto 9.8.Coexistence of distant genetic forms in population, is actually what polymorphism means. Parental polymorphism could be surveyed using SSR markers which are becoming standard DNA markers for plant genome analysis and are now being used in marker assisted breeding.DNA extraction and purification of sodicity tolerance( pH 9.8) CSR11 and MI48 varieties was done. Forty three SSR primers and five saltol SSR primers were used to screen the parental varieties. Among SSR primers, 26 showed polymorphism between the parents and seventeen were found to be monomorphic. Among five Saltol AAE primers, two primers showed monomorphism and three showed polymorphism.